更新時間:2016-06-14
SC瓊脂基礎,年中放價銷售了,低價、*、優惠價供應,微生物培養基折扣價提供,BD培養基、Gibco培養基、Amresco培養基、OXOID培養基等國內外培養基,我們傾情提供。
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SC瓊脂基礎 | Sulfite Cycloserine Agar Base | 250g | 用于產氣莢膜梭菌的計數和培養,需天極D-環絲氨酸和50%卵黃乳液(ISO標準) |
用途:用于產氣莢膜梭菌的計數和培養,需天極D-環絲氨酸和50%卵黃乳液(ISO標準)
SC瓊脂基礎 配方:(g/L) | |||||||||||||||||
胰酪蛋白胨 大豆胨 酵母浸粉 亞硫酸氫鈉 檸檬酸鐵銨 瓊脂 PH值 | 15.0g 5.0g 5.0g 1.0g 1.0g 13.0g 7.6±0.2 | ||||||||||||||||
原理: | |||||||||||||||||
胰酪蛋白胨、大豆蛋白胨和酵母浸粉提供碳氮源、維生素和生長因子;亞硫酸氫鈉和檸檬酸鐵銨用于檢測硫化氫的產生,使菌落中心呈黑色;瓊脂是培養基的凝固劑;D-環絲氨酸抑制非梭菌的細菌。 | |||||||||||||||||
用法: | |||||||||||||||||
稱取本品 40 克,加熱溶解于1000ml蒸餾水中, 121℃高壓滅菌15分鐘,冷卻至44-47℃時,加入10ml過濾除菌的4%D-環絲氨酸溶液,混勻,傾入無菌平皿,備用。 | |||||||||||||||||
質量控制: | |||||||||||||||||
在36±1℃培養18-24小時。
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